【摘 要】
：
In this study,the Comparison of Elsholtzia volatile compounds from the Stem(with Leaf)and Flower was acquired.The volatile compounds of these two parts from Elsholtzia aerial parts was respectively an
【机 构】
：
Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical
论文部分内容阅读
In this study,the Comparison of Elsholtzia volatile compounds from the Stem(with Leaf)and Flower was acquired.The volatile compounds of these two parts from Elsholtzia aerial parts was respectively analyzed by a rapid and convenient static headspace injection technique coupled with gas chromatography–mass spectrometry and the relative contents of each constituent between the two different parts were determined by peak area normalization.61 compounds were identified in the Stem(with Leaf)and Flower,among which 39 components simultaneously exist.47 and 53 compounds were separated and identified in the volatile compounds from the different parts of Elsholtzia,respectively.There were differences among some volatile constitutes of the two parts,but the main constitutes were all Elsholtzia ketone,Caryophyllene,3-Octanol and Dehydroelsholtzia ketone.The most abundant components in the Stem(with Leaf)included Elsholtzia ketone(84.20%),Caryophyllene(4.3%)and 3-Octanol(3.11%),while Elsholtzia ketone(88.03%),Caryophyllene(3.33%)and 3-Octanol(1.53%)were the main components in the Flower.8 single constituents were identified in the Stem(with Leaf)volatiles including 3-Heptanone and Linalool,while 14 constituents only including 2-Methylbutanoic acid and Perillen have been found in the Flower volatiles.Elsholtzia ketone was the main compound,and its concentration was significantly higher than other substances(>80%of the total oils in Elsholtzia).The study provided an important scientific base for the further utilization of Elsholtzia resources and may be helpful for systematically understanding the constituents of volatile compounds of Elsholtzia.
其他文献
鉴定六神曲中的主要化学成分，分析产地、工艺和原料对六神曲主要成分的影响。采用HMBC、H-HCOSY、TOCOSY 等多种NMR 技术鉴定六神曲中主要成分，利用多元统计分析36 批六神曲的主要成分差异。从六神曲中鉴定出琥珀酸(1)、乳酸(2)、甘油酸(3)、甲氧基乙酸(4)、甲氧基乙酸铵(5)脂肪酸(6)、甘油(7)、长链烯烃(8)、α-D-果糖(9)、α-D-葡萄糖(10)和β-D-葡萄糖(11
建立RP-HPLC 同时测定姜黄-桂枝药对中肉桂酸、桂皮醛、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素5 种主要成分.采用色谱柱SHIMADZU VP-ODS C18(250mm×4.6 mm,5 μm)；流动相为乙腈(A)-0.1％冰醋酸(B)；梯度洗脱；流速1 mL/min；检测波长330 nm；柱温30℃；进样量10 μL.在该色谱条件下,桂皮醛、肉桂酸、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄
优选三种姜汁(干姜煎汁、生姜煮汁、生姜榨汁)制备工艺，为后续不同姜汁炮制具体药物提供参考.以6-姜辣素和8-姜酚含量的综合评分为评价指标，通过 L9(34)正交试验考察不同因素对制备工艺的影响.采用HPLC 测定6-姜辣素与8-姜酚的含量，流动相乙腈-水(71∶29)，检测波长280nm.干姜煎汁制备工艺为取干姜适量煎煮三次，每次一小时，依次加入12、10、8 倍量的水.合并汁液，过滤，浓缩至约为
采用电子鼻技术,比较分析微波法和清炒法对南葶苈子清炒品的影响,为南葶苈子炮制工艺提供参考.净制南葶苈子,分别以微波法、清炒法进行加热炮制,得到微波炮制品和清炒品,用电子鼻技术对其进行分析鉴定.FOX4000 电子鼻和HERACLES气相电子鼻两种,经过校正后对样品逐个采集信息,每个样品变换位置采集3 次信息,数据处理方法采用了Alpha soft 软件,具有多种数据分析方法.主成分分析全部样品的主
测定半夏不同炮制品(法半夏、姜半夏和清半夏)中尿苷和肌苷的含量.方法：采用高效液相色谱法；Ultimate XB-C18 色谱柱(4.6 mm × 250 mm,0.45 μm)；流动相：甲醇-水；流速：1.0 mL/min；检测波长：260 nm；柱温：30℃；采用梯度洗脱法测定半夏不同炮制品中尿苷和肌苷的含量.结果：尿苷含量大小依次是姜半夏＞法半夏＞清半夏＞生半夏；肌苷含量大小依次是：姜半夏＞
考察经不同炮制方法得到的仙茅饮片中仙茅苷、苔黑酚葡萄糖苷的含量变化。采用高效液相色谱法对仙茅苷、苔黑酚葡萄糖苷进行含量测定。酒炙品、吴茱萸汁炙品、盐制品、酒蒸品、米泔水制品中仙茅苷含量均高于生品，以米泔水制品为最高；酒炙品、姜汁炙品、吴茱萸汁炙品、酒蒸品中苔黑酚葡萄糖苷含量均高于生品，以姜汁炙品为最高。酒炙法、吴茱萸汁炙法、酒蒸法均能提高仙茅中仙茅苷和苔黑酚葡萄糖苷的含量，以酒炙法最为显著。
金银花的加工方法是影响其质量稳定性的关键因素；本文通过阅读大量有关金银花产地加工方面的文献，总结出金银花主要的产地加工方法，并对每一种方法的特点进行了归纳，旨在为金银花生产中选择合理的加工方法提供理论依据。
研究博落回的化学成分。采用硅胶柱层析以及制备液相色谱法进行分离纯化，并根据波谱数据鉴定化合物结构。从博落回叶95％的乙醇提取物中分离鉴定1个生物碱，鉴定为：4-去甲氧基-6-甲氧基山椒酰胺。化合物1 为新化合物。
测定半夏不同炮制品(法半夏、姜半夏和清半夏)中鸟苷和腺苷的含量.采用高效液相色谱法；Ultimate XB-C18 色谱柱(4.6mm × 250mm,0.45μm)；流动相：甲醇-水；流速：1.0 mL/min；检测波长：260 nm；柱温：30℃；采用梯度洗脱法测定半夏不同炮制品中肌苷、鸟苷和腺苷的含量.半夏生品中鸟苷和腺苷含量均高于各炮制品.半夏生品中鸟苷和腺苷含量最高,不同炮制方法对半夏中
对黑顺片、炮附片的HPLC 色谱图进行比较分析,探究砂烫对黑顺片中酯型生物碱类成分的影响。以乙醚—二氯甲烷(1∶4)混合溶液为提取溶剂,采用高效液相色谱法,Agilent Extend-C18 色谱柱,梯度洗脱,检测波长240nm 检测样品。炮附片与黑顺片色谱图比较,有7 个共有的峰,砂烫后有1 个峰消失,新增5 个峰。黑顺片炮制前后其化学成分发生质变和一定程度的量变,现行药典的检测指标不足以评价