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One case A 36-year-old woman was admitted who plan to undergo mitral valve replacement.Cardiopulmonary bypass was done routinely.The heart could not resuscitate after release of aortic clamp.The aortic valve replacement would be done.4:1 hyperkalemic cold blood cardioplegia perfused through aorta root antegradely,and the dosage was 25ml/kg.The retrograde cardioplegia perfusion was performed through coronary sinus per 30min.