An amino acid analyzer (AAA) method for the analysis of free amino acids (FAA) in extracts of L.vannamei meat was developed.The analysis was performed on Hitachi L-8900 amino acid analyzer with a Column The column[60 mm×4.6 mm (i.d.)]within 108 mins.The correlation coefficient values (R2 > 0.9990) indicated good correlations between the investigated amino acids concentrations and their peak areas within the test ranges;The LOD and LOQ were 0.10~6.31 μmol/L and 0.30~20.36 μmol/L,respectively;The recoveries ranged from 83.6% to103.6% and the overall relative standard deviations for intra-and inter-day were lower than 2.42%.The results showed that this method is facile,reliable,and accurate for the analysis of FAA in L.vannamei meat.The method was also applied to quantitatively determine FAA profiles in L.vannamei samples.Results showed that the major FAAs were Glu(158.94 mg/100 g),Gly(921.24 mg/100 g),Ala(284.47 mg/100 g),Arg(656.80 mg/100 g),Pro(mg/100 g),AspNH2(50.30 mg/100 g),GluNH2(76.25 mg/100g),which comprised 92.2 % of the total content.Trp,Ala and Orn were all increased with storage time.But Orn was increased more significant and linearly correlated with storage time than Trp and Ala during chilled storage,Orn was useful as a potential index for freshness and decomposition ofL.vannamei.