Integral membrane proteins (IMPs) play a critical role in mediating a host of biological and physiological processes including receptor function,transduction,ion transport and propagation of signaling cascades,and therefore they are often regarded as molecular targets for drug discovery.However,IMPs analysis remains challenging due to the highly hydrophobic nature,resulting in poor solubility in aqueous buffer and tendency of aggregation.In our recent work,various new methods for membrane proteome analysis have been developed.