【摘 要】
：
UV-B辐射作为一种重要的环境信号对植物生长发育的许多方面均有显著影响,以前的研究已显示UV-B辐射能诱导植物激素乙烯的生物合成,且乙烯产生主要是通过调节乙烯合成过程中的关键酶——ACC合成酶的活性来调节乙烯的生成,而酶的活性主要取决于编码此酶的基因在rnRNA水平上的表达量多少；同时,大量证据也表明极低剂量的UV-B辐射就能启动依赖于UV-B受体——UVR8的UV-B专一信号通路,并依赖此信号通
【机 构】
：
陕西师范大学生命科学学院,西安710062
论文部分内容阅读
UV-B辐射作为一种重要的环境信号对植物生长发育的许多方面均有显著影响,以前的研究已显示UV-B辐射能诱导植物激素乙烯的生物合成,且乙烯产生主要是通过调节乙烯合成过程中的关键酶——ACC合成酶的活性来调节乙烯的生成,而酶的活性主要取决于编码此酶的基因在rnRNA水平上的表达量多少；同时,大量证据也表明极低剂量的UV-B辐射就能启动依赖于UV-B受体——UVR8的UV-B专一信号通路,并依赖此信号通路调控着植物基因表达、UV-B耐受性和植物生长等多种生理过程.但是,目前并不清楚UV-B辐射诱导乙烯生物合成是否与ACS基因表达有关,ACS基因家族中哪些参与了UV-B辐射诱导植物叶片乙烯的生物合成,UVR8信号通路是否参与UV-B辐射调控ACS基因表达以及乙烯生物合成等.本论文以模式植物拟南芥的野生型和各种突变体的叶片为材料,借助半定量PCR (RT-PCR)和实时定量PCR(qRT-PCR)技术,主要探讨了不同剂量UV-B辐射调控了哪些种类的ACS基因表达,UVRg-COP1-HY5/HYH在不同剂量UV-B辐射诱导ACS基因表达中的作用以及乙烯在不同剂量UV-B辐射诱导UVR8信号通路成员HY5和HYH基因表达中的作用.本文得到以下主要实验结果：以野生型拟南芥叶片为材料,当UV-B辐射强度在0.02 W·m-2和0.5W·m-2时,不同程度地诱导了ACS2,ACS4,ACS6,ACS7,ACS8,ACS11的基因表达,且不同基因的适宜处理时间不一样；UVR8-COP1-HY5/HYH在不同剂量UV-B辐射调控ACS基因表达中发挥着一定的作用,但在不同ACC合酶中的作用有所不同；乙烯信号转导途径参与了UV-B辐射对UVR8信号通路成员HY5和HYH基因表达的诱导.
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