【摘 要】
:
For quantitative real-time PCR (qRT-PCR) analysis of gene expression in plants,the key prerequisite that determines the accuracy of results is the selection of appropriate reference gene(s).Goji (Lyci
【机 构】
:
宁夏农林科学院农业生物技术研究中心,银川750002
论文部分内容阅读
For quantitative real-time PCR (qRT-PCR) analysis of gene expression in plants,the key prerequisite that determines the accuracy of results is the selection of appropriate reference gene(s).Goji (Lycium barbarum) is a multi-branched shrub belonging to the Solanaceae family.Despite its outstanding stress resistance and economic value,no systematic screening or evaluation of reference gene(s) in L.barbarum has been performed,mainly due to the lack of its whole genome data.In this work,we identified 18 candidate reference genes from the transcriptomic sequencing data of 14 samples obtained from the leaves,flowers,and fruits of L.barbarum at different developmental stages and under drought stress condition.The expression stability of these candidate genes was rigorously analyzed using qRT-PCR and four different statistical algorithms: geNorm,BestKeeper,NormFinder and RefFinder.We found that two novel reference genes LbCML38 and LbRH52 showed the most stable expression.On the contrary,traditionally used reference genes such as LbGAPDH,LbHSP90 and LbTUB showed unstable expression in the tested samples.
其他文献
GHRL基因在哺乳动物、鸟类、鱼类及两栖类中广泛存在,与之对应的生长素(Ghrelin,GHRL)是一种来自胃的辛酰基化生长激素释放肽,具有调节食欲、胃肠功能、心血管系统和能量平衡等作用。一般认为生物个体的生长素的水平同个体的生长速度存在一定正向关联。从与生长性状相关的基因中筛选与目标性状关联的分子标记,能够为传统的选育工作提供助力。单核苷酸多态性位点(SNP)标记作为第三代分子标记在生物体内分布
建立渔船在自由航行和起网两种工况下的轴系扭振当量系统,介绍了不同轴段柔度的计算方法。利用compass软件进行轴系扭转振动计算分析,得到在正常发火和一缸熄火情况下各轴段的合成应力曲线和相关部件扭矩曲线。结果 表明,该渔船轴系最大扭振合成应力和部件的扭矩均小于许用值,可为渔船轴系设计提供参考。
The chemical composition and antioxidant activity of essential oils and methanol extracts of stems,needles and berries from Juniperus rigida were studied.The results indicated that the yield of stems
Ethnopharmacological relevance: Juniperus rigida is used as Tibetan and Mongolian medicine in China for the treatment of rheumatoid arthritis,nephritis,brucellosis and other various inflammatory disea
Notopterygium incisum and N.oviforme,belonging to the family Apiaceae,are the two endangered perennial herbs endemic to China.In recent years,due to excavation,as well as the expansion of human activi
Mytilus galloprovincialis foot protein type 5 (Mgfp-5) can be used as medical adhesives or underwater environments due to its significant adhesive ability and biodegradable properties.To obtain enough
The gone of diphosphomevalonate decarboxylase was cloned successfully from Euphorbia helioscopia L.,named as EhMDC (accession number: KP995936).The full length cDNA of EhMDC was 1653 bp,and it contain
Salvia miltiorrhiza Bunge is an ancient herb that has been widely used for the treatment of cardiovascular disease,while Salvia castanea Diels f.tomentosa Stib is served as a substitute product.1 g/L
Nuclear migration and positioning are fundamental processes of eukaryotic cells.To date,no evidence for a direct connection between nucleus migration and programmed cell death (PCD) has been described
The basic region/leucine zipper (bZIP) transcription factors (TFs) play vital roles in plants responses to abiotic stresses.However,little is known about the function of bZIP genes in Oxytropis ochroc