【摘 要】
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目的 Differentiation of T Follicular helper(Tfh)cells are enhanced in patients with acute asthma exacerbation,but their associations with the expression of B cells and IgE level are not clear.In this st
【机 构】
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The central hospital of Wuhan
【出 处】
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2016中华医学会呼吸病学年会暨第十七次全国呼吸病学学术会议
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目的 Differentiation of T Follicular helper(Tfh)cells are enhanced in patients with acute asthma exacerbation,but their associations with the expression of B cells and IgE level are not clear.In this study,we aim to explore the correlations between Tfh cells and B cells,as well as plasma lgE,IgG1 levels.方法 Peripheral blood samples were collected from forty-two plasma patients with acute exacerbation and forty-eight matched controls.Tfh cells(CD4+CXCR5+)and B cells(CD19+)were examined by flow cytometry,while plasma IgE,IgG1 and IL-21 level were measured by enzyme-linked immunosorbent assay(ELISA).结果 The percentage of Tfh cells(p＜0.05),B cells(p＜0.01)in peripheral blood,plasma IgE(p＜0.001),IgG1(p＜0.001)and IL-21(p＜0.001)levels were all increased in patients with acute asthma exacerbation compared with health controls.The percentage of Tfh cells was associated with B cells(r=0.67,P＜0.001)and plasma IgE level(r=0.56,P＜0.001),while no significant correlation between the percentage of Tfh Cells and IgG1(r=0.22,P=0.33)in asthma patients with acute exacerbation.Similarly,plasma IL-21 level was associated with B cells(r=0.84,P＜0.001)plasma lgE level(r=0.60,P＜0.01),with no significant correlation between the plasma level of IL-21 and IgG1(r=0.09,P=0.68)in asthma patients with acute exacerbation.结论 Tfh cells may participate in the airway inflammation reaction through promoting the differentiation and IgE class switching of B cells in asthma patients with acute exacerbation.
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