Activity and Structure of H+-ATP Synthase from the Chloroplast

来源 :2008中国深圳蛋白质和多肽科学大会 | 被引量 : 0次 | 上传用户:cxcqjf
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  ATP synthase is an important enzyme for the living organisms by oxidative phosphorylation as the energy source.The driving force for ATP synthesis is an electrochemical gradient of protons(△pH) and/or sodium ion(△Na+) generated initially by electron transfer complexes across the mitochondrial,chloroplast,or bacterial membrane.In chloroplast system,only proton can be the driving force for ATP synthesis based on recent studies.We discuss about if Na+ ion and Li+ ion being the driving force of H+-ATP synthase by the method of chemiluminescence.In the analysis data,Na+ ion can be the driving force for ATP synthesis,and its initial rate is 3.5 s-1 under our experimental condition.Although the initial rote is slower than 200 s-1 which driven by proton (A pH 4.4),it is faster than 0.7 s-1 which driven by Na+ ion of Propionigenium Modestum.
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