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Protein analysis by HPLC is of great interest to both academic research and the development of therapeutic biomolecules.For proteins in the range of 20-150 kDa in size, reversed phase chromatography (RP-HPLC) is often the technique of choice due to its ability to separate proteins and peptides that share a high degree of homology and also for its ability to resolve biomolecules from complex sample matrices.In most cases, the optimal choice of chromatographic media for such difficult separations is one that has the highest chromatographic efficiency.Until recently, the dominant trend in the evolution of HPLC media has been the gradual shift to smaller particle sizes, a trend which culminated (for now) in the relatively widespread acceptance of columns packed with sub-2 micrometer particles.