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The matrix is very important to ensure the perfect performance of chromatography.Cellulose beads are one kind of main chromatographic matrix for bio-molecule separation.However,the traditional preparation method based on the cellulose xanthate viscose is technologically complex,and brings some environmental burdens which involve the use of hazardous chemicals and the exhaust of some toxic gases [1].Based on the previous work [2-5],ionic liquid would be used as the non-derivatizing solvent to prepare porous cellulose beads with the method of water-in-oil suspension and cooling/ethanol regeneration,and some porogenic agents were used to enlarge the pore size of cellulose beads.