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Background: Hepatitis B vaccine immunization is the only effective measure for prevention of Hepatitis B Virus (HBV) infection.However many defects, including the weak response or even nonresponse in common population and only T helper cell type 2 (Th2) immunity, have been reported.Human beta defensin 2 (hBD2), possessing features of antibiotics and immunity regulation, is probably a promising natural immune adjuvant.