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Microorganisms continue to be a productive focus for much of marine natural products research.However,most of the studies were drug-oriented,and the potential of marine-derived microorganisms and their active metabolites serving for crop protection have not received enough attention.In this study,98 fungi isolates associated with sponge Hymeniacidon perleve collected from the Bohai Sea were screened for insecticidal activities with brine shrimp lethality assay (BSA).