Quantitative assessment of the myelin water content in the brain can substantially improve our understanding of white matter diseases such as multiple sclerosis.In this study,in vivo myelin water content was estimated using T2* relaxation with multislice acquisitions in magnetic resonance imaging (MRI).The main advantages of using T2* relaxation are (1) a low specific absorption rate (SAR),which is especially beneficial for imaging at high field strengths,(2) a short first echo time (~2 ms) and short echo spacing (~1 ms),which allows for the acquisition of multiple sampling points during the fast decay of the myelin water signal,and (3) fast multislice acquisitions.High-resolution and multislice myelin water fraction (MWF) maps were obtained in a clinically acceptable scan time at 3T.