Potential of white rot fungus Trametes pubescens biomass was explored for biosorption of azo dye Congo Red.The biomass immobilized by sodium alginate or D201 enhanced the sorption capacity for Congo Red approximately 1.5-fold of the free biomass.Dye uptake was favored by acidic conditions at pH 2.0 with increasing initial dye concentration up to 100 mg/L using less biomass at room temperature and agitation speed.