BSS1 as a negative regulator for brassinosteroid signaling that was identified by chemical biology u

来源 :The 2nd International Brassinosteroid Conference(第二届国际油菜素甾醇会 | 被引量 : 0次 | 上传用户:shulili1987
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Brassinosteroids (BRs) play important roles in plant development and response to environmental cues.BIL1/BZR1 is a master transcription factor in BR signaling,but the mechanisms that lead to the finely tuned targeting of BIL1/BZR1 by BRs are unknown.Here,we identified BSS 1 (BRZ-SENSITWE-SHORT HYPOCOTYL 1) as a negative regulator of BR signaling by chemical biology analysis using Brz,a specific BR biosynthesis inhibitor.The bss1-1D mutant,which overexpresses BSS1,presented a Brz-hypersensitive phenotype in hypocotyl elongation.BSS1 encodes a BTB-POZ domain protein with ankyrin repeats,known as BLADE ON PETIOLE 1 (BOP1),which plays an important role in regulating leaf morphogenesis.The bss1-1D mutant exhibited an increased accumulation of phosphorylated BIL1/BZR1 and a negative regulation of BR-responsive genes.
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