Surface enhanced Raman scattering(SERS)nanoparticles can be utilized as optical labeling nanoprobes similar to fluorescent dyes and quantum dots for bioimaging with additional advantages of the fingerprint vibrational signal as a unique optical code and the ultra-narrow linewidth for multiplexing.Typically,their SERS efficiencies reach maximal values at the resonant condition,when the excitation wavelength overlaps with the localized surface plasmon resonance wavelength preferably in the near-infrared biological window.However,the on-resonant photo-generated heat may inevitably disturb or even destroy a biological sample or induce the photo-bleaching of SERS signals during the imaging process.In this talk we will present our recent off-resonant SERS nanoprobes for bioimaging and biosensing.