【摘 要】
：
Gene expression profiling using microarrays combined with qPCR has created new possibilities for the molecular characterisation of breast cancer.We have completed whole genome microarray analysis of >
【机 构】
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Biomarkers & Targeted Therapies School of Pharmacy & Pharmaceutical Sciences Trinity College Dublin
【出 处】
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(BITs 3rd Annual World Cancer Congress-2010, Breast Cancer C
论文部分内容阅读
Gene expression profiling using microarrays combined with qPCR has created new possibilities for the molecular characterisation of breast cancer.We have completed whole genome microarray analysis of >100 archived breast tumour biopsies (with normal breast tissue as control).Additionally, using cancer cell line models, we optimised methods enabling us to establish that cancer cells apparently selectively secrete mRNAs and microRNAs (miRNAs).Furthermore, RNA was successfully isolated and analysed from pre-surgery serum from recently diagnosed breast cancer patients, from their breast tumour and matched normal biopsies, and from post-surgery serum.Similarly, analysis of a cohort of specimens from triple-negative breast cancers have been analysed compared to controls.miRNA profiles were assessed using TaqMan low density arrays (TLDAs), allowing the co-analysis of 667 miRNAs.Our extensive range of in vitro models enables the functional relevance of key gene products be assessed using si/shRNA and cDNA over-expression technologies, followed by a battery ofphenotype-assessing assays.This presentation will summarise novel findings from our exosomes/RNA and functional genomics analysis; highlighting both the challenges and the successes achieved thus far.Acknowledgements: This work was supported by the Health Research Board of Ireland (RP/2006/77: LOD); Trinity College Dublin Start-Up Funding for New Academics; and Science Foundation Ireland (08/SRC/B 1410).
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