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A highly efficient demulsifying stain,S-XJ-1,was isolated from petroleum-contaminated soil and identified as Alcaligenes sp.by 16S rRNA gene and physiological test.It achieved 96.5%and 49.8%of emulsion breaking ratio in W/O and O/W kerosene emulsion within 24 h,respectively,and also showed 95%of water separation ratio in oilfield petroleum emulsion within 2 h.