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Microfluidics and micrometer-scale total analysis systems (μTAS) have been experienced a fast development in last two decades.They take the advantages of downscaling laws to achieve better performances,such as high resolution and sensitivity of separation and detection; low cost and consumption of samples and reagents; shorter reaction times and faster heat transfer for analysis; and adaption of automation for commercial uses.In this work,we focus on micro-fabrication technologies and its practical applications in environmental pollutants detection.We choose MVLN cells (a bioluminescent MCE-7-derived cell line to study the modulation of estrogenic activity) as indicator cells,exposing them to various kinds of estrogens and antagonists.Channels on different layers with specific design conduct different chemicals from inlets to culture wells and stimulated real conditions in environment.The performance of whole-Teflon chips were examined through cells growth status and activity under the effect of estrogens and antagonists by detect their luminance intensity.The whole-Teflon chips were proved to be capable of providing a static culture environment to cells and generating concentration gradient of pollutants.