The rabies virus (RABV) has less genetic material and RABV replication mainly depends on host cell machinery for energy.The proliferation of the RABV in the host cell is the virus-host cell interaction and regulation.The results in our previous research showed that many host protein joined the process and integrated into the RABV virion during invading, reproduction, package and budding stage.On behalf of this background, this experiment focused on the RABV virion released from the host cell infected with RABV.Firstly, the mature RABV virion was purified with deferential centrifugation and sucrose gradient centrifugation.Then the virion was proceeded with proteomics analysis through lyqiud chromatogram and mass spectrum tandem method.The results show that 60 host proteins, along with five virus-encoded structual proteins, have been identified in the purified RABV particles, which can be classified into ten categories according to the function.To screen the special host protein, immunogold labeling, western blot and confocal laser technology were used to obtain the host protein and the location of the host protein in the RABV virion.At last RNA interference, the expression of cell line, confocal laser technology, the coprecipitation of immunity and Real-time PCR technology were used to explore the key role of the host protein in the life cycle of RABV.It will be helpful to reveal the pathogenic mechanism of RABV.