【摘 要】
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Gene-modified human cells and mice are important tools for studying gene function in vivo.Before 2010, gene-modified human cells and mice were mainly genera
【出 处】
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The 3rd Canton Nucleic Acids Forum(第三届广州核酸国际论坛)
论文部分内容阅读
Gene-modified human cells and mice are important tools for studying gene function in vivo.Before 2010, gene-modified human cells and mice were mainly generated by gene targeting, which was time-consuming and technique-intensive.In 2010, artificial nucleases based genome-editing tools were developed to facilitate genetic modified human cells and mice production.After introduced into cells, these artificial nucleases can induce double strand break (DSB) at target sites.DSBs will be repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR), resulting in frame-shift mutation or precise gene modification respectively.Genome editing in mouse embryos provides an easier and more efficient way to generate mutant mice.So far, several different kinds of artificial nucleases have been developed, including ZFN, TALEN and CRISPR/Cas systems.The application of CRISPR/Cas technology for genetic studies in human cells and mice will be discussed here.
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