Diabetic cardiomyopathy (DCM) is cardiac disease caused by diabetes but independent of hypertension, coronary artery disease (CAD), with high incidence, great harm, poor prognosis.Studies on DCM in recent decades shown that several factors such as metabolic disturbance, small vessel disease, myocardial fibrosis, cardiac autonomic dysfunction, and insulin resistance have been implicated as putative mechanisms leading to DCM.However, that pathological mechanisms of DCM on the molecule level is far from been fully understood, due to the complicity of DCM. Our current study means to characterize the pathophysiological mechanisms of DCM, and the pharmacological mechanisms of a particular herbal medicine (effective in treating DCM).We successfully build an STZ-induced DCM rat model, and then we collect microarray data and some physiological data from four groups of samples: Normal, Disease, Disease with herbal-medicine-treating and Disease with chemical-medicine-treating. After raw-data processing, we combined different approaches to comprehensively interpret the data: identify genes showing differential expression, or different connectivity, or strongly correlated to physiological character; construct weighted gene co-expression networks and determine significant modules. Gene-set enrichment analysis, pathway and PPI network mapping were also done. As results, besides verifying some mechanisms already known being related to DCM,We also identified several novel pathways and genes, and targets of that herbal medicine. All these results have implications in providing guidance and advice on pathological analysis and drug development of DCM.