【摘 要】
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Using tissue immunohistochemistry technique,the present study investigated the effects of subcutaneous (s.c.) bee venom (BV) administration on the expression of three major NMDA receptor subunits (NR1
【机 构】
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Institute for Biomedical Sciences of Pain, Capital Medical University, Beijing 100069, China
【出 处】
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中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
Using tissue immunohistochemistry technique,the present study investigated the effects of subcutaneous (s.c.) bee venom (BV) administration on the expression of three major NMDA receptor subunits (NR1,NR2A,NR2B) in rat lumbar spinal cord,S 1 area and hippocampus.Subsequent to s.c.BV injection (100 ug/50 ul) into the hind paw,the animal exhibited a typical behavioral expression of persistent pain and hyperalgesia,whereas s.c.injection of equal volume of normal saline produced little effect.The coronal sections of lumbar spinal cord and brain were sequentially prepared at 4 h after BV or saline administration.Immunostaining was then performed and positive labeling was visualized by treatment with diaminobenzidine (DAB) and hydrogen peroxide.Quantifications of NMDA receptor subunits expression were finished by comparisons between two sides of the hindpaw or comparisons between BV-treated and saline-treated groups.
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