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Thl cell-mediated immune responses at the site of active infection are important to restrict the growth of M.tuberculosis (MTB) and for the spontaneous resolution of patients with tuberculous pleurisy(TBP).In the present study, we found that without any stimulation, CD4+ T cells in pleural fluid cells(PFCs) from patients with TBP expressed significantly higher levels of CD69 than PBMCs from patients with tuberculosis (TB) or healthy donors.CD4+CD69+ T cells expressed T-bet and IL-12Rβ2.