【摘 要】
：
The GP ofhantavirus is a main candidate protein for the HTV genetically engineered vaccine, but its immunogenicity is not satisfactory.The chimeric genes GnS0.7 and GcS0.7 from hantavirus can take the
【机 构】
：
Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University,
【出 处】
：
中国免疫学会第九届全国免疫学学术大会
论文部分内容阅读
The GP ofhantavirus is a main candidate protein for the HTV genetically engineered vaccine, but its immunogenicity is not satisfactory.The chimeric genes GnS0.7 and GcS0.7 from hantavirus can take the advantages of both GP and NP.In this research the adenoviral vector pShuttle was modified with a hybrid CAG promoter/enhancer to improve the expression of the fusion proteins GnS0.7 and GcS0.7.The multi-CTL epitopes gene from S segment was integrated in the recombinated vector to enhance the cellular responses, and a HSP70 C-terminal gene was added as adjuvantas.
其他文献
研究背景:F-box蛋白是一类广泛存在于真核生物中,含有F-box结构域的蛋白家族,在泛素-蛋白酶体途径(ubiquitin-proteasome pathway,UPP)中因特异识别底物蛋白而参与细胞周期调控、转录调控、细胞凋亡、细胞信号转导等生命活动.我们课题组通过siRNA技术筛选影响VSV病毒诱导的Ⅰ型干扰素或炎症因子的泛素连接酶,发现了F-box家族的成员Fbxo21可能在天然免疫中发挥
The NLRP3 inflammasome is the most characterized inflammasome activated by cellular infection or stress, which is responsible for the maturation of proinflammatory cytokines IL-1β and IL-18.The precis
The Hantaan virus (HTNV) is a rodent-borne virus that causes hemorrhagic fever with renal syndrome (HFRS) in China and Asia.HTNV can be transmitted by inhaling aerosolized urine, droppings, or saliva
目的:观察肾脏缺血再灌注(Ischemia Reperfusion,IR)后miR-146的表达变化,并探究导致其表达变化的原因.方法:以C57BL/6小鼠为实验对象,采用先后夹闭双侧肾蒂30min的方法构建肾脏热IR损伤模型.于再灌注后0h、1h、3h、6h、12h、24h、48h、72h采下腔静脉血检测血肌酐及尿素氮,同时依次摘取小鼠双侧肾脏进行病理检查,并利用实时荧光定量逆转录聚合酶链反应(
The Interferon regulatory factor (IRF) family encodes transcription factors that play important roles in immune defense,stress response, reproduction, development, and carcinogenesis.Despite the chara
目的:运用HSP70/CD80 DNA疫苗治疗急性哮喘小鼠,并研究该过程中Th 1/Th2/Treg/Th 17再平衡的作用机制.方法:用鸡卵清蛋白(OVA)致敏构建急性小鼠哮喘模型,肌肉注射DNA疫苗进行治疗.测定气道反应性,血清中IgE的含量,检测肺组织的病理学改变;ELISA检测支气管肺泡灌洗液中细胞因子IFN-γ、IL-4、TGF-β和IL-17的表达;Real-time PCR检测肺组织
研究背景:痘苗病毒(Vaccinia virus,W)属于痘病毒科(Poxviridae)正痘病毒属(Orthopoxvirus),具有宿主范围广泛、外源基因容量大、胞质复制等优势,具备成为高效安全基因工程载体的潜质.痘苗病毒目前已广泛应用于基因工程疫苗载体和外源基因表达系统等研究领域.但传统的痘苗病毒疫苗株在应用过程中常伴有发热、丘疹、脑炎等不同程度的副反应,尤其对于一些免疫抑制和患慢性疾病的患
目的:通过检测肿瘤干细胞标记物CD133、Nestin(神经巢蛋白)在脑转移瘤组织中的表达情况,探讨二者在脑转移瘤的形成及侵袭发生发展过程中的作用,了解脑转移瘤中肿瘤干细胞的相关生物学特性.方法:应用免疫组织化学的方法对52例经手术切除的脑转移瘤标本,10例原发灶肿瘤标本,进行CD133、Nestin蛋白表达的测定,同时取10例正常人脑组织(颅脑损伤患者行内减压切除术的脑组织)标本作为对照,对每张
The CD4 binding site (CD4BS) of the HIV-1 envelope glycoprotein (Env) contains epitopes for broadly neutralizing antibody (nAb) and is the target for the vaccine development.However, the CD4BS core co
研究背景:miRNA在肿瘤的发生发展过程中发挥重要作用,多种miRNA在不同肿瘤恶性转化中的促癌/抑癌作用已被证实,但不同miRNA在特定肿瘤中发挥功能的机制仍需深入探索.目的:探讨RNA-221/222(MiR-221/222)对胶质瘤细胞生物学功能的影响和下游调节靶基因,观察MiR221/222在胶质瘤生物学行为中所起的作用.方法:通过荧光实时定量聚合酶链反应(qRT-PCR)检测14例胶质瘤