【摘 要】
：
An in vivo, highly selective method has been established to assay α-glucosidase inhibitory activity.The principle of the method was to measure the maltose in mouse ansa intestinalis homogenate at some
【机 构】
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Shenyang Pharmaceutical University China
【出 处】
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2013第三届内分泌与代谢大会暨2013第二届糖尿病大会
论文部分内容阅读
An in vivo, highly selective method has been established to assay α-glucosidase inhibitory activity.The principle of the method was to measure the maltose in mouse ansa intestinalis homogenate at some given time points after a certain quantity maltose was injected into the amphi-ligated enteric cavity.Then, HPLC-UV with 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP) pre-column derivatization was used to measure the maltose levels in ansa intestinalis homogenate.Two α-glucosidase inhibitors acarbose and berberine were used to test α-glucosidase inhibitory activity.The results showed this new method is feasible.
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