【摘 要】
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Background: von Willebrand factor (vWF) is a potent regulator of angiogenesis, tumor growth, and metastasis.Yet, the expression pattern of vWF in human gastric cancer (GC) tissues and its relation to
【机 构】
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Institute of Immunology, Third Military Medical University, Chongqing 400038, PR China
【出 处】
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中国免疫学会第九届全国免疫学学术大会
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Background: von Willebrand factor (vWF) is a potent regulator of angiogenesis, tumor growth, and metastasis.Yet, the expression pattern of vWF in human gastric cancer (GC) tissues and its relation to clinicopathological features of these cases remains unknown.Methods: Tumor and 5 cm adjacent non-tumoral parenchyma specimens were collected from 99 patients with GC (early stages Ⅰ/Ⅱ and late stages Ⅲ/Ⅳ), and normal specimens from 32 healthy controls (reference group).Plasma vWF antigen (vWF∶Ag) and vWF activity were assessed by ELISA.The role of vascular endothelial growth factor (VEGF) in differential vWF expression was investigated using cultured human umbilical vein endothelial cells (HUVECs).vWF and VEGF protein and mRNA expression levels were investigated by qRT-PCR, western blotting and immunohistochemistry (IHC) respectively.The correlation of IHC-detected vWF expression with patient clinicopathological characteristics was analyzed.
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