论文部分内容阅读
Axon-wrapping myelin sheath is essential for proper functioning of the nervous system.Myelin is formed from membranous sheets that are elaborated by oligodendrocytes(OLs)in the central nervous system(CNS).Although much is known about the development and migration of OL and its progenitor cell in vitro and in vivo,how CNS myelin is dynamically formed through OL processes is still unclear.To examine the process of CNS myelination in vivo,we performed long-term time-lapse confocal imaging of the same myelin sheath segment on Mauthner-axon in the ventral spinal cord of intact zebrafish larvae during 2 – 7 days post-fertilization(dpf)with intervals of 2 – 12 hr.Individual OLs were mosaically labeled by membrane-tethered red fluorescent protein(mRFP)under the control of zebrafish Sox10 regulatory DNA.