We have recently shown that transplantation of embryonic limb progenitor cells can stimulate limb regeneration in the non-regenerating forelimb of adult frogs (Xenopus laevis).Here we report that this approach can also be used to induce regeneration in the mouse digits.We have optimized method for transplanting limb progenitor cells onto the mouse middle phalanx (P2) stumps and identified a panel of growth factors that can promote proliferation of embryonic limb progenitor cells and maintain the differentiation potential of limb progenitor cells in vitro.Transplantation of embryonic limb progenitor cells, together with combinations of growth factors, can significantly enhance digit regeneration after P2 amputations in the adult mice.The transplanted cells have mainly contributed to the bone lineage.Our results indicate that limb progenitor cell transplantation can be utilized to treat limb and digit injuries.