Initiated by Prof.J.S.Chiao in 1950s, our laboratory focused on the development of bacterial fermentation technology up to 1980s and our knowledge exploration research was mainly based on forward genetics-based gene mapping and biochemistry-based functional analysis of fermentation related enzymes.With the introduction and development of powerful tools of molecular biology and genomics since 1990s, bacterial physiology has been studied based on a broader spectrum of complete nature blueprints employing recombinant technology in industrial strains,both in vitro and in vivo, for better and broader applications.Thus, microbial physiology was transformed to systems biology based on omics analyses, which facilitated the quantitative understanding of the nature of biological processes and the improvement of production of biological molecules via microbial metabolic and protein engineering.Since the beginning of this century, bottom-up forward engineering strategy and robust "standardized" technology platforms have been explored via sophisticated innovation geared by the emerging area of research that enables both the design and construction of novel artificial biological pathways,organisms or devices, or the redesign of existing natural biological systems.Although Synthetic Biology is a long march for the rebirth of life science and biotechnology in the fire of Convergence innovation, by integrating well-coordinated dedicated research teams, it is going to grow up and to revolutionize our understanding of life and our capability of industrialization of biology for the wealth and health of human beings.