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The T-box transcription factor T-bet is a key regulator for the lineage commitment in CD4 Th1 cells and CD8 T cells by activating the hallmark production of interferon-γ, and its expression level is linked to autoimmune diseases. T-1993C and T-1514C polymorphisms in the TBX21 gene (encoding T-bet) promoter can affect transcription activity.We investigated the distributions of these functional polymorphisms in 84 adult patients with type 1 autoimmune hepatitis (AIH-1) and 318 healthy controls. The influence of the T-1993C polymorphism on transcription and its functional effect by luciferase reporter and EMSAs, and flow cytometric analysis of intracellular T-bet, IFN-? And IL-4 expression in activated CD4+ T cells. Intracellular T-bet staining of polarized CD4 Thl cells from healthy controls corresponding to T-1993C genotypes were analyzed by flow cytometry.