目的 体外Tk基因突变试验常用于检测受试物的遗传毒性。试验中的代谢活化条件通常采用添加化学诱变剂诱导的大鼠肝脏制备的微粒体酶，目前尚缺少检测单一亚型酶对受试物毒性影响的实验体系。细胞色素P450酶2E1(CYP2 E1)参与多种致癌物的代谢活化，本实验应用可稳定表达CYP2E1并且具有TK基因杂合性缺失(TK+/-)的人淋巴母细胞株h2E1V2及其亲本细胞株AHH-1细胞进行Tk基因突变实验，考察
Introduction: High intensity focused ultrasound (HIFU) is gaining wide acceptance among the scientific committee.
To evaluate the use of respiratory "head out" plethysmographys for use as a respiratory function assessment tool in rats as part of the respiratory system assessment required under the ICH S7A guideli
GLP的实施水平会影响到动物实验的实施是否适当。对动物实验结果有影响的因素的恒定或设定条件的维持、控制对保证实验的重现性具有重要影响。科学的动物饲育管理,以及完善的操作记录,能够保证良好的实验质量。饲育环境的各种指标必须尽可能在基准值范围内。另外,动物从接收、检疫、治疗、实验等环节,都必须进行卫生的饲育管理。动物相关的各种数据也必须获取。实验设施中日常的动物饲育管理必须制定必要的SOP,不应该有遗
The aim of this article is to examine the toxic effects of hexabromocyclododecanes(HBCDs) on astrocytes primary culture and conditional culture solution of HBCDs-treated astrocytes on the survival of
目的 通过激光诱导猴眼底黄斑区脉络膜新生血管(CNV)渗漏和生长，建立与人脉络膜新生血管类似的动物模型，对拟用于治疗脉络膜新生血管相关疾病药物的药效学进行评价。方法 采用眼科激光光凝仪围绕猴黄斑中心凹进行光凝造模，光凝后2～3周，进行荧光素眼底血管造影检查，筛选出造模成功的动物，根据造模效果分为模型对照组、阳性对照组、药物低、中、高剂量组。分别于给药前、给药后14和28 d进行眼底彩色照相、荧光素
Green tea has a considerable amount of polyphenolic catechins and has been promoted to have beneficial effects on health including protective effects against oxidative stress.Acetaminophen (APAP) is a
Tetrandrine is an alkaloid with a remarkable pharmacological profile that we previous reported causes hepatotoxicity through mitochondrial dysfunction.Here, we demonstrate that cytochrome P450 (CYP450
OBJECTIVE Cisplatin-induced nephrotoxicity has been characterized previously in animals and humans.Two studies were conducted in cynomolgus macaques to assess the nephrotoxicity of cisplatin and to co
目的 建立DSS诱导大鼠急性炎症性肠病(IBD)模型,研究维生素D和维生素E对急性IBD的治疗作用及探讨其作用机制.方法 ①将雄性Wistar大鼠分为如下7组,正常对照,DSS模型对照,泼尼松龙(1 mg·kg.·d-1)阳性对照,维生素D(50 ng·d-1),维生素E(2 g·kg-1·d-1),维生素D(50 ng·d-1) +E(2 9·kg-1·d-1)联合,以及泼尼松龙(1 mg·kg