【摘 要】
:
目的 研究白果内酯在大鼠体内的吸收和分布特征.方法 健康SD大鼠,雌雄各半,随机分组后分别灌胃(7,14,28 mg/kg)和静注(14 mg/kg)给予白果内酯水溶液后,采用液相色谱-串联质谱(LC-MS/MS)法测定血浆和组织中白果内酯的浓度,用DAS2.1.1软件计算主要药动学参数.采用SPSS16.0软件对试验结果进行统计分析.结果 灌胃给药后,大鼠血浆中低、中、高三个剂量组白果内酯的Cm
【机 构】
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沈阳药科大学;药物代谢与动力学重点实验室,沈阳 110016
【出 处】
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第十届全国药物和化学异物代谢学术会议暨第三届国际ISSX/CSSX联合学术会议
论文部分内容阅读
目的 研究白果内酯在大鼠体内的吸收和分布特征.方法 健康SD大鼠,雌雄各半,随机分组后分别灌胃(7,14,28 mg/kg)和静注(14 mg/kg)给予白果内酯水溶液后,采用液相色谱-串联质谱(LC-MS/MS)法测定血浆和组织中白果内酯的浓度,用DAS2.1.1软件计算主要药动学参数.采用SPSS16.0软件对试验结果进行统计分析.结果 灌胃给药后,大鼠血浆中低、中、高三个剂量组白果内酯的Cmax分别为2324、3810和5631 μg/L, AUC0-4分别为280354,514990和794212μg·min/L,Tmax分别为32.5、43.33和27.5 min,T1/2分别为55、66和121 min.白果内酯的绝对生物利用度为97.6%.组织分布试验结果表明,灌胃给药0.25、0.5、1和3h后白果内酯在多数组织器官中均有分布,在小肠、胃、肾、肝、心和肺中的浓度较高,在脑中也有分布.给药7h后各组织中的药物浓度均显著下降.结论 大鼠灌胃白果内酯后在胃肠道吸收迅速和充分,较快分布于全身各组织,没有组织蓄积现象.AUC0-t和Cmax的增长和剂量之间没有明确的线性关系.
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