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Cyclodextrins (CDs) was cyclic oligosaccharides consisting of six or more α-(l,4)-linked D-glucopyranose units.The β-cyclodextrin molecule had secondary 2-and 3-hydroxyl groups lining the mouth of the cavity and primary 6-hydroxyl groups at the rear of the molecule.The cavity permitted inclusion of hydrophobic portions of the solute molecules.The monolithic column was prepared in a 100 mm × 4.6 mmi.d.stainless steel chromatographic column tube by an in situ polymerization technique.The porogenic solvent was a mixture of toluene and heptane,functional monomer and cross-linker were glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) respectively,and 2,2′-azobisisobutyronitrile (AIBN) was initiator.Scanning electron microscope (SEM) showed that the monolithic material was homogeneous with highly interconnected pores,forming a network of channels (Figure 1).The monolith skeleton in the dry state was characterized by mercury intrusion porosimeter,and showed a specific surface area of 5.11 m2 g-1,average pore size of 1.12 μm,and a total porosity of 65.6%.Oligo-β-cyclodextrin was coupled to the stationary phase for a rapid separation and determination of puerarin from Radix pueradae isoflavonoids.The polymerization conditions,including the ratio of functional monomer and cross-linker,the porogenic solvent,polymerization time and temperature,coupling concentration,temperature and time,were investigated to obtain an efficient monolith.The chromatographic performance of the monolith was evaluated in high performance liquid chromatography (HPLC).This study has shown that the oligo-β-cyclodextrin bonded monolithic column has a potential application in the rapid separation and determination of natural products.