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Aim: PLK1 is a serine/threonine kinase that regulates cell cycle and is overexpressed in many tumors.Our study aimed to investigate the biological functions and mechanisms of PLK1 in metastasis of esophageal squamous cell carcinoma (ESCC).Methods: The ESCC cell overexpressed PLK1 was screened by RT-PCR and Western blot.The effective PLK1siRNA fragment was screened by Western blot.