【摘 要】
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Screening of toxicity, biological mechanisms and biomarkers as well as the establishment of detection methods are key points of translational toxicology.Internal chemical exposome analysis (ICEA) refe
【出 处】
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2016(第二届)毒性测试替代方法与转化毒理学(国际)学术研讨会暨有害结局路径(AOP)与风险评估培训会议
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Screening of toxicity, biological mechanisms and biomarkers as well as the establishment of detection methods are key points of translational toxicology.Internal chemical exposome analysis (ICEA) refers to the detection of chemicals (toxicants, drugs and nutrients) and their metabolites in human samples (body fluids, tissues and cells) with the integrated use of liquid chromatography tandem mass spectrometry, gas chromatography tandem mass spectrometry and inductively coupled plasma mass spectrometry.We firstly established the ICEA platform covering more than 1000 kinds of toxicants, drugs and nutrients and their metabolites, and found that urinary exposure levels of phytoestrogens, phenolic compounds, polycyclic aromatic hydrocarbon metabolites, heavy metals and metalloids were associated with male infertility and adverse birth outcomes, and therefore the toxic effects of chemicals and their metabolites on reproductive and developmental function as well as biomarkers of exposure were identified.Furthermore, we found exposure to these chemicals significantly changed the concentrations of nutrients and their metabolites in the body fluids, tissues and cells, which mediates the toxic effects, thus revealing biomarkers of effect for toxicants as well as the reproductive and developmental toxicity mechanisms.ICEA reveals combined effects of complex chemical internal exposure on human health, and it provides an important method for toxicant exposure early assessment and susceptible individual identification in the population as well as prevention of toxic effects.
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