Organophosphates (OPs) widely exist in ecosystem as toxic substances,and it is high desire to develop a sensitive and rapid analytical method in the field.In this talk,a genetically engineered Escherichia coli (E.coli) strain surface displayed mutant organophosphorus hydrolase (OPH) (S5) with improved enzyme activity using N-terminal of ice nucleation protein as anchoring motif was constructed.Further,a novel microbial biosensor for the rapid monitoring of p-nitrophenyl-substituted organophosphates (OPs) compounds based on glass carbon electrode (GCE) modified with both ordered mesopore carbons (OMCs) and cell surface-expressedOPH (OPH-bacteria/OMCs/GCE) will be highlighted.Compared to OPH-bacteria modified GCE (OPH-bacteria/GCE),the OPH-bacteria/OMCs/GCE not only significantly enhanced the current response but also reduced the oxidation overpotential towards oxidizable p-nitrophenol (p-NP) which was the hydrolysis product of p-nitrophenyl-substituted OPs.Under the optimized experimental conditions,the current response was linear with paraoxon concentration in 0.05-25 μM.The low limit of detection was evaluated to be 9.0 nM for paraoxon (S/N=3).Thus,a highly specific,sensitive and rapid microbial biosensor was established and it holds great promise for on-site detection of trace p-nitrophenyl-substituted OPs.Therefore,the enzyme-displayed bacterial cell can not only be used directly without further enzyme extraction and purification,but also it improves the stability of the enzyme.Therefore,through the use of genetic manipulation means,bacterial whole-cell would be engineered to serve as novel methods for analyte detection,which could be more efficient and cost-effective in combination of novel nanostructures.