Single nucleotide polymorphisms(SNP)has a number,density,genetic stability,ease of genotyping,and large-scale,automated analysis of the advantages of it are subject to a number of scholars sought after.In the EST sequences of SNP(EST-SNP)has been more mature,polyploid plants of Brassica napus SNP development and application,especially suitable for the more widely used means of detection but slow progress.To this end,this study is to establish a set of SNP detection system for routine analysis.