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Objective: Determination of P.hookeri in different medicinal parts of the total saponins content and compare the differences in different parts.Method: With Ursolic acid as a reference substance, the spectrophotometer was used to measure the total content of saponins in different medicinal parts of Pterocephalus hookeri.Result: There is a good linear relationship within at 0.0582 ~ 0.2038 mg range of ursolic acid absorbance content with the correlation coefficient r = 0.9991.The average recovery was 97.06 % and RSD = 93 %.Conclusion: The average content of total saponin of P.hookeri aerial parts is up to 5%.Therefore, it is suggested that, on the condition of resource protection and assurance of curative effect, the aerial part of its medicine should be adopted.