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  5. The Comparison of Regulation of CyclinA Gene of a New Type HPV16 E7 Gene from Hubei Province of Chin

The Comparison of Regulation of CyclinA Gene of a New Type HPV16 E7 Gene from Hubei Province of Chin

来源 :2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会) | 被引量 : 0次 | 上传用户：NobelHsu
【摘 要】
：
Objective:The comparison of carcinogenicity of HPV 16 HB variant E7 and HPV 16 WE7.Methods:To construct a luciferase reporter gene plasmid which was under the control of cyclinA promoter.HPV16 HB vari
【作 者】
：
Xinxing Wu Liqin Zhu 
【机 构】
：
Virology Institution,Medical School of Wuhan University, Wuhan City, China
【出 处】
：
2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
【发表日期】
：
2005年12期
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论文部分内容阅读
　　Objective:The comparison of carcinogenicity of HPV 16 HB variant E7 and HPV 16 WE7.Methods:To construct a luciferase reporter gene plasmid which was under the control of cyclinA promoter.HPV16 HB variant E7 and HPV16 WE7 were separately cloned into pCD3.1.Cotransfections were performed by using a cyclinA reporter plasmid and each expression as indicated(HPV-16WE7,HPV-16HBE7) or empty vector as control.To control for transfection,cell were cotransfected with the pRL-cytomegalovirus plasmid.Luciferase activity was assayed.Results:A two-fold increase of cyclinA promoter activity was observed when HPV16 HB variant E7 was co-transfected in comparison of an empty vector.
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