Introduction: Dental pulp stem cells(DPSCs)-mediated dental pulp regeneration is considered as a promising method for treatment of deep caries with pulpitis,enabling conservation and restoration of teeth.However,mesenchymal stem cells(MSCs)senescence is an adverse factor for the perspective of cell-based therapies.In this study,we investigated the characteristics and expression profiles of DPSCs from young and old donors.Methods: DPSCs from young and old donors were cultured in differentiation medium and then detected their differentiation potential.Long noncoding RNA(LncRNA)microarray assays and bioinformatic analysis was performed to investigated the differences in lncRNA and mRNA expression profiles between DPSCs from young and old donors.Results: We found that DPSCs from young donors exhibited more powerful proliferation ability,osteogenic and adipogenic differentiation potentials compared with DPSCs from old donors.In DPSCs from young donors,numerous lncRNAs were significantly up-regulated(n=389)or down-regulated(n=172)compared to DPSCs from old donors.Furthermore,304 mRNAs were differentially expressed including 247 up-regulated genes and 57 down-regulated genes in DPSCs from young donors.Bioinformatic analysis identified that several pathways may be associated with DPSCs characteristics,such as Cell cycle,RNA transport,etc,and revealed that NFYB,GTF2B and NR3C1 were identified as core regulatory factors and FR249114,FR299091 and ENST00000450004 were identified as core lncRNAs.Conclusions: Our results indicated that senescence impaired the proliferation and differentiation potentials of DPSCs,donor age is an important factor which affected their usage for tooth regeneration.We also provided insight into the mechanisms responsible for the senescence in DPSCs.