【摘 要】
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polycyclic aromatic hydrocarbons(PAHs)are a kind of important procarcinogens.Formation of hydroxy polycyclic aromatic hydrocarbons(OH-PAHs)from PAHs via phase Ⅰ metabolism was reported to be the key p
【机 构】
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Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation,East China Institute of Technology,
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polycyclic aromatic hydrocarbons(PAHs)are a kind of important procarcinogens.Formation of hydroxy polycyclic aromatic hydrocarbons(OH-PAHs)from PAHs via phase Ⅰ metabolism was reported to be the key process of the detoxification of PAHs.By now,the conversion of PAHs to OH-PAHs was considered to be a unidirectional process.However,in this work,for the first time,we found OH-PAHs can convert to PAHs reversibly under physiological conditions.This finding would give scientists new insights into the metabolism of PAHs.In the experiment,1-hydroxy pyrene(1-OHP),the most widely used biomarker of internal dose of PAHs exposure,was used as a typical OH-PAHs to investigate the conversion of OH-PAHs to PAHs.ESI-MS was used to monitor the conversion process.Experimental results showed that the conversion of OH-PAHs to PAHs can occur in the human body physiological tissue without enzymes participation,and the conversion enhanced with the decrease of pH and the increase of temperature.Besides,a radical involved mechanism was proposed for conversion of OH-PAHs to PAHs according to Density Functional Theory(DFT)calculation.
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