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目的:探讨舒郁胶囊对慢性应激诱导的经前期综合征(PMS)肝气郁证大鼠的治疗作用及谷氨酸(Glu)与γ-氨基丁酸(GABA)比值在其作用机制中的改变。方法:根据旷场实验及糖水偏好实验大鼠随机分为空白组、模型组、氟西汀组、舒郁胶囊组与柴胡皂苷组。药物治疗作用在给药4d后通过体质量、旷场实验及糖水偏好实验进行评价。微透析收集海马细胞外液,HPLC检测透析液中的Glu及GABA含量。结果:与空白组比较,模型组糖水偏好系数、旷场总得分及体质量显著下降(P<0.01,P<0.05),舒郁胶囊组与模型组相比该3指标均显著上升(P<0.05)。结论:舒郁胶囊可能通过抑制中枢神经系统海马中Glu与GABA比值下降治疗PMS肝气郁证。
Objective: To investigate the therapeutic effect of Shuyu capsule on chronic stress-induced premenstrual syndrome (PMS) in rats with liver-qi stagnation and the effect of the ratio of glutamate (Glu) to γ-aminobutyric acid (GABA) change. Methods: According to the open field experiment and sugar preference experiment, rats were randomly divided into blank group, model group, fluoxetine group, Shuyu capsule group and saikosaponin group. The effect of drug treatment was evaluated by body weight, open-field test and sugar preference test after 4 days of administration. The hippocampal extracellular fluid was collected by microdialysis and the content of Glu and GABA in the dialysate was detected by HPLC. Results: Compared with the blank group, the preference coefficient of sugar water, the total score of open field and the body weight of the model group were significantly decreased (P <0.01, P <0.05), while those of Shuyu capsule group and the model group were significantly increased (P < 0.05). Conclusion: Shuyu capsule may decrease the ratio of Glu to GABA in the hippocampus of the central nervous system to treat PMS.