Vibrio parahaemolyticus,one of the most important foodbome pathogens in many maritime Asian countries,is frequently associated with the consumption of seafood.Thirty eight strains of V.parahaemolyticus were isolated from seafood in Hebei province of China.Resistance to 13 antibiotics was determined using broth microdilution methods.These strains were typed by pulsed-field gel electrophoresis (PFGE) technique with short pre-processing following SfiI digestion and a typing scheme was generated.