【摘 要】
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Existence of porcine endogenous retrovirus (DERV) hinders pigs to be used in clinical xenotransplantation toalleviate the shortage of human transplants. Chinese miniature pigs are potential organ don
【机 构】
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Laboratory for Viral Safety of National Centre of Biomedical Analysis, Institute of Transfusion Medi
【出 处】
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2011东莞第二届国际小型猪学术论坛暨大型实验动物生物医药研究应用研讨会
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Existence of porcine endogenous retrovirus (DERV) hinders pigs to be used in clinical xenotransplantation toalleviate the shortage of human transplants. Chinese miniature pigs are potential organ donors for xenotransplantation in China.However, so far, an adequate level of information on the molecular characteristics of PERV from Chinese miniature pigs has notbeen available. We described here the cloning and characterization of full-length proviral DNA of PERV from Chinese Wuzhishanminiature pigs inbred (WZSP). Full-length nucleotide sequences of PERV-WZSP and other PERVs were aligned and phylogenetictree was constructed from deduced amino acid sequences of env. The results demonstrated that the full-length proviral DNA ofPERV-WZSP belongs to gantmaretrovirus and shares high similarity with other PERVs. Sequence analysis also suggested thatdifferent patte＞bs of LTR existed in the same porcine germ line and partial PERV-C sequence may recombine with PERV-Asequence in LTR.
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