Road networks are essential components of widespread socioeconomic infrastructure development.However,road ecology has emerged as a hot topic in the past decade as the ecological impacts of road networks and its scaling effects have been poorly studied.The Longitudinal Range-Gorge Region (LRGR) is characterized by its rich biodiversity,high ecological vulnerability and rapid road network construction.The aim of this study was to examine multi-scaled impacts of road networks and topography on regional ecosystems in LRGR.The independent effects of road network density and their interactive effects with topographic factors (i.e.,elevation,slop & aspect) on ecosystems were analyzed by using spatial correlation and ANOVA with scaling from 2×2 km to 20×20 km.The results showed that the correlation between road network density and ecosystem transformation is determined by the road network category as correlation values decreased with upgraded road systems.In addition,topographic factors showed scale independent impacts,and their interactive effects with topography occurred at most scales.Further,the independent effects of higher-grade road network categories were much more distinct,while the topographically interactive effects of those lower-grade road network categories are notable at most scales,indicating that their extensive ecological impacts are prone to being topographically altered.