Aging is a physiological phenomenon caused by genes,environment and diets that changes during the body function declines.Many factors contribute to aging,such as free radical theory,telomere theory and mitochondrial DNA damage.Especially the free radical theory focus on that the cytomembrane,nucleic acid,protein and other,enzymes are damages caused by free radical accumulation.The mechanism of aging is radically divided into two categories,spontaneous aging and passive aging.The former focused on the aging process controlled by genes,while the latter preferred to that negative substances accumulation in vivo accelerated the ageing process,such as reactive oxygen species and inflammation.Recently,scientists pointed out that balance of intestinal flora affect health and disease,for example obesity,hypertension,irritable bowel syndrome and other diseases.The commensal bacterium changes along with aging process and it has a different compositing in the elder and the younger.The gastrointestinal flora can also influence active of centre nervous system.The gut-brain axis was the concept based on that.The gastrointestinal flora was related with nervous system in aging process.The healthy gut flora can delay aging of animals by reducing inflammation and promoting health which provides a new ideal for disease curing.Prebiotics could selectively stimulate one or many species probiotics and be good for health.Specific Prebiotic (SP) using in this study consisted of dietary fiber and polysaccharides from plant.Our research focused on evaluating anti-aging effects of prebiotics via interaction with gut flora.SP also increases thermos tolerance ability of worms.We also research on the indigenous populations of the microbial community in worms by high throughput sequencing technology.