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A myxobacteria strain, EGB, was isolated from saline soil with the yeast as the sole carbon and nitrogen resource.Strain EGB was identified as Corallococcus sp.according to its morphological and physiological characteristics and phylogenetic analysis of the 16S rRNA gene sequence.Strain EGB secreted a β-glucanase into the culture media which showed high hydrolytic activity against yeast cell wall.The glucanase was a β-1,6-glucanase as tested by periodate oxidation.The growth of phytopathogenic fungi Verticillium dahliae,Fusarium oxysporum and Ustulaginoidea virens was inhibited when co-cultured with Corallococcus sp.EGB on plates.The hyphae of these fungi were seriously lysed.The crude enzyme could efficiently lyse cell walls of the three pathogens fungi as observed by lactophenol-cotton blue staining.Results indicated that β-1,6-glucanase played important roles in the control of pathogenic fungi.These characteristics showed that strain EGB with the lytic activity may be an potential biocontrol agent for Cotton Verticillium wilt, Cucumber Fusarium Wilt and Rice False Smut.