The effects of CO2-enhanced high pressure processing (CO2+HPP) on the inactivation of dioxide Escherichia coli and Staphylococcus aureus were investigated in this study,and the possible mechanism was proposed on cellular level.A self-designed inflation device was used for CO2 inflation,and HPP treatment was performed with a Stansted Fluid Power Model FPG7100 High Pressure Food Processor.The total plate count method was used to count the microbial population.