【摘 要】
：
利用双层平板法分离获得了一株L.casei ATCC 393烈性噬菌体,命名为Lcb,噬菌体由多面体头部和非收缩性尾部组成,为Siphoviridae科,B1类.该噬菌体基因组不含粘性末端,其包
【机 构】
：
东北农业大学动物医学学院,哈尔滨市香坊区木材街59号
论文部分内容阅读
利用双层平板法分离获得了一株L.casei ATCC 393烈性噬菌体,命名为Lcb,噬菌体由多面体头部和非收缩性尾部组成,为Siphoviridae科,B1类.该噬菌体基因组不含粘性末端,其包装机制属满头包装,即pac-型.噬菌体的其潜伏期和裂解期分别为75min和45min,裂解量为16PFU/mL.噬菌体Lcb对酸、碱有一定的耐受性,在pH3～12范围内均维持一定的存活率；对乙醇和异丙醇的耐受性较低；对温度敏感；该噬菌体在0℃时吸附率最小(20％),30℃时最大(83.51％)；Ca2+和/或Mg2+的添加能促进噬菌体对宿主菌的吸附；噬菌体Lcb可以吸附到经热灭活处理的细胞上,但吸附率下降约40％.噬菌体Lcb的表型及基因型特征均表明该噬菌体为一株新的干酪乳杆菌噬菌体.
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